Magnesium Regulates the Circadian Oscillator in Cyanobacteria

@article{Jeong2019MagnesiumRT,
  title={Magnesium Regulates the Circadian Oscillator in Cyanobacteria},
  author={Young M. Jeong and Cristiano L. Dias and Casey O. Diekman and Helene Brochon and Pyonghwa Kim and Manpreet Kaur and Yong-Sung Kim and Hye-In Jang and Yong-Ick Kim},
  journal={Journal of Biological Rhythms},
  year={2019},
  volume={34},
  pages={380 - 390}
}
The circadian clock controls 24-h biological rhythms in our body, influencing many time-related activities such as sleep and wake. The simplest circadian clock is found in cyanobacteria, with the proteins KaiA, KaiB, and KaiC generating a self-sustained circadian oscillation of KaiC phosphorylation and dephosphorylation. KaiA activates KaiC phosphorylation by binding the A-loop of KaiC, while KaiB attenuates the phosphorylation by sequestering KaiA from the A-loop. Structural analysis revealed… Expand
CikA, an Input Pathway Component, Senses the Oxidized Quinone Signal to Generate Phase Delays in the Cyanobacterial Circadian Clock
TLDR
This work introduced a new cyanobacterial circadian clock mixture that includes an input component in vitro that reemphasizes the mechanistic input functionality of CikA, contrary to other reports that focus only on its output action. Expand
Purification of GST-Fused Cyanobacterial Central Oscillator Protein KaiC
The cyanobacterial circadian clock is the most well-understood and simplest biological time-keeping system. Its oscillator consists of three proteins: KaiA, KaiB, and KaiC. When combined together inExpand
The Circadian Clock—A Molecular Tool for Survival in Cyanobacteria
TLDR
The understanding of the cyanobacterial circadian clock is highlighted and how it functions as a molecular chronometer that readies the host for predictable changes in its surroundings is discussed. Expand
Bayesian Modeling Reveals Ultrasensitivity Underlying Metabolic Compensation in the Cyanobacterial Circadian Clock
TLDR
This study takes a data-driven kinetic modeling approach to characterizing the interaction between KaiA and KaiC in the cyanobacterial circadian oscillator and understanding how the oscillator responds to changes in cellular metabolic conditions. Expand
Nitrogen Sources and Iron Availability Affect Pigment Biosynthesis and Nutrient Consumption in Anabaena sp. UTEX 2576
TLDR
Insight is presented into developing optimal Anabaena culture for efficient operations of bioproduction and wastewater bioremediation with cyanobacteria and variations on pigmentation and nutrient uptake were attributed to changes in phycocyanobilin biosynthesis, light-induced oxidation of carotenoids, and urea-promoted peroxidation. Expand
Micronutrient Inadequacy in Short Sleep: Analysis of the NHANES 2005–2016
TLDR
Overall, it is demonstrated that short sleep is associated with increased nutrient inadequacy, emphasizing the possible need for dietary supplementation. Expand
Dietary Approaches to Stop Hypertension (DASH) Score and Its Association with Sleep Quality in a National Survey of Middle-Aged and Older Men and Women
TLDR
It was found that the DASH diet score was inversely related to poor sleep-related daytime dysfunction adjusted by age, sex, demographic and socio-economic factors. Expand

References

SHOWING 1-10 OF 39 REFERENCES
CikA Modulates the Effect of KaiA on the Period of the Circadian Oscillation in KaiC Phosphorylation
TLDR
Insight is given into how to reconstitute the cyanobacterial circadian clock in vitro by the addition of an input pathway component, and how this affects circadian oscillations by directly interacting with the oscillator components. Expand
Detecting KaiC phosphorylation rhythms of the cyanobacterial circadian oscillator in vitro and in vivo.
TLDR
Here, a detailed protocol to obtain pure recombinant proteins from Escherichia coli to construct a robust cyanobacterial circadian oscillator in vitro is provided and a protocol that facilitates analysis of phosphorylation states of KaiC and other phosphorylated proteins from in vivo samples is presented. Expand
Structural basis of the day-night transition in a bacterial circadian clock
TLDR
The metamorphic properties of KaiB, a protein that adopts two distinct folds, and the post–adenosine triphosphate hydrolysis state of KaiC create a hub around which nighttime signaling events revolve, including inactivation of KaiA and reciprocal regulation of the mutually antagonistic signaling proteins, SasA and CikA. Expand
A protein fold switch joins the circadian oscillator to clock output in cyanobacteria
TLDR
It is shown that KaiB flips between two distinct three-dimensional folds, and its rare transition to an active state provides a time delay that is required to match the timing of the oscillator to that of Earth’s rotation. Expand
The day/night switch in KaiC, a central oscillator component of the circadian clock of cyanobacteria
TLDR
KaiA and KaiB likely act by shifting the dynamic equilibrium of the A-loops between exposed and buried states, which shifts the balance of autokinase and autophosphatase activities of KaiC. Expand
A sequential program of dual phosphorylation of KaiC as a basis for circadian rhythm in cyanobacteria
TLDR
Double phosphorylation converted KaiC from an autokinase to an autophosphatase, whereas complete dephosphorylation had the opposite effect, and these mechanisms serve as the basis for cyanobacterial circadian rhythm generation. Expand
Ordered Phosphorylation Governs Oscillation of a Three-Protein Circadian Clock
TLDR
A mathematical model constrained by experimental data quantitatively reproduces the circadian period and the distinctive dynamics of the four phosphoforms shown in an ordered pattern arising from the intrinsic autokinase and autophosphatase rates of KaiC and their modulation by KaiA. Expand
Oxidized quinones signal onset of darkness directly to the cyanobacterial circadian oscillator
TLDR
It is shown that pulses of oxidized quinones reset the cyanobacterial circadian clock both in vitro and in vivo, causing an abrupt oxidation of the plastoquinone pool in vivo. Expand
Reconstitution of Circadian Oscillation of Cyanobacterial KaiC Phosphorylation in Vitro
TLDR
The self-sustainable oscillation of KaiC phosphorylation in vitro is reconstituted by incubating KaiC with KaiA, KaiB, and adenosine triphosphate and the enigma of the circadian clock can now be studied in vitro by examining the interactions between three Kai proteins. Expand
Daily magnesium fluxes regulate cellular timekeeping and energy balance
Circadian clocks are fundamental to the biology of most eukaryotes, coordinating behaviour and physiology to resonate with the environmental cycle of day and night through complex networks ofExpand
...
1
2
3
4
...