Maggots and wound healing: an investigation of the effects of secretions from Lucilia sericata larvae upon the migration of human dermal fibroblasts over a fibronectin‐coated surface

@article{Horobin2005MaggotsAW,
  title={Maggots and wound healing: an investigation of the effects of secretions from Lucilia sericata larvae upon the migration of human dermal fibroblasts over a fibronectin‐coated surface},
  author={Adele J Horobin and Kevin M. Shakesheff and David Idris Pritchard},
  journal={Wound Repair and Regeneration},
  year={2005},
  volume={13}
}
Lucilia sericata larvae, or greenbottle fly maggots, placed within chronic wounds have been observed to remove necrotic tissue and infection. They are also believed to actively promote granulation tissue formation. Interactions between fibroblasts and the surrounding extracellular matrix play a crucial role in tissue formation, influencing fibroblast proliferation, migration, and tissue remodeling. For example, the strength of cell adhesion to surfaces coated with extracellular matrix… 
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Promotion of human dermal fibroblast migration, matrix remodelling and modification of fibroblast morphology within a novel 3D model by Lucilia sericata larval secretions.
TLDR
Mechanisms by which maggots enhance tissue formation within wounds may be via the promotion of fibroblast motility, acceleration of extracellular matrix remodelling and coordination of cellular responses.
Greenbottle (Lucilia sericata) Larval Secretions Delivered from a Prototype Hydrogel Wound Dressing Accelerate the Closure of Model Wounds
TLDR
The present results suggest that controlled delivery of ES as a follow‐up to maggot debridement therapy may be an effective therapeutic option for stimulation of tissue regeneration in wound management.
Development of bioactive electrospun scaffolds suitable to support skin fibroblasts and release Lucilia sericata maggot excretion/secretion
TLDR
The fabrication of a novel bioactive scaffold that can be loaded with Lucilia sericata maggot excretion/secretion for wound therapy, and which also provides structural stability for mammalian cell-growth and migration is described.
The Effect of Salivary Gland Extract of Lucillia Sericata Maggots on the Human Dermal Fibroblasts Proliferation
TLDR
The obtained results suggest that medicinal maggots may speed up the wound healing process through the stimulation of fibroblast migration, and further detailed research is needed to fully elucidate the substance (s) responsible for fibrlob last stimulation and migration.
Does maggot therapy promote wound healing? The clinical and cellular evidence
  • Y. Nigam, C. Morgan
  • Biology, Medicine
    Journal of the European Academy of Dermatology and Venereology : JEADV
  • 2016
TLDR
The clinical evidence which links maggots and improved wound healing is summarized, and recent scientific studies which examine and identify the role of maggot, particularly individual components of maggot secretions, on specific cellular aspects of wound healing are identified.
Wound debridement potential of glycosidases of the wound‐healing maggot, Lucilia sericata
TLDR
Investigation of the glycosylation profiles of wound slough/eschar from chronic venous leg ulcers and the complementary presence of glycosidase activities in first‐instar excretions/secretions (ES1) and to define their specificities suggest that maggot Glycosidases remove sugars from slough /eschar proteins.
The effect of Lucilia sericata larval excretion/secretion (ES) products on cellular responses in wound healing
TLDR
This review summarizes the relevant literature on ES‐mediated effects on the cellular responses involved in wound healing and suggests that larva excretion/secretion (ES) products can facilitate the healing processes directly without the need of direct contact with the larvae.
The transcriptional responses of cultured wound cells to the excretions and secretions of medicinal Lucilia sericata larvae
TLDR
Although ES had no direct effect on wound cell phenotypes it did partially reduce the immune response to bacterial LPS exposure, consistent with the profile of transcriptional responses that were dominated by modulation of immune response genes.
Molecular events underlying maggot extract promoted rat in vivo and human in vitro skin wound healing
  • Pei-Nan Li, Hong Li, Decheng Lv
  • Biology, Medicine
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
  • 2015
TLDR
It is shown that both body extract and secretion/excretion of maggots contain favorable wound healing elements and that the enhancement of TGF‐beta/Smad3 and STAT3 signaling activities may be the main molecular effects of maggot extracts on the wound tissues.
A Jonah-like chymotrypsin from the therapeutic maggot Lucilia sericata plays a role in wound debridement and coagulation.
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References

SHOWING 1-10 OF 49 REFERENCES
Maggots and wound healing: an investigation of the effects of secretions from Lucilia sericata larvae upon interactions between human dermal fibroblasts and extracellular matrix components
TLDR
Fibroblast–ECM interactions exert considerable influence upon new tissue formation in greenbottle fly larvae, partaking in a dynamic feedback loop with the fibroblasts that produce it.
Growth effects of Phaenicia sericata larval extracts on fibroblasts: mechanism for wound healing by maggot therapy.
  • P. Prete
  • Biology, Medicine
    Life sciences
  • 1997
Fibronectin provides a conduit for fibroblast transmigration from collagenous stroma into fibrin clot provisional matrix.
TLDR
The functional requirements for fibroblast transmigration from collagen-rich to fibrin-rich matrices, such as occurs in early wound healing, have been partially defined using an in vitro paradigm of this important biologic process.
Degradation of extracellular matrix components by defined proteinases from the greenbottle larva Lucilia sericata used for the clinical debridement of non‐healing wounds
Background  Larvae of the greenbottle fly Lucilia sericata are used routinely for the clinical treatment of difficult necrotic and infected wounds. Degradation by proteinases contained in larval…
Proteinases and Tissue Remodeling
The term tissue remodeling describes transient or permanent changes in tissue architecture that involve breaching of histological barriers such as basement membranes, basal laminae, and interstitial…
Attachment of intrinsically and extrinsically aged fibroblasts on collagen and fibronectin.
Fibroblasts cultured from venous ulcers display cellular characteristics of senescence.
Defective extracellular matrix reorganization by chronic wound fibroblasts is associated with alterations in TIMP-1, TIMP-2, and MMP-2 activity.
TLDR
The data suggest that the impaired ability of chronic wound fibroblasts to reorganize extracellular matrix in vitro is related to decreased levels of active matrix metalloproteinase-2 and matrix meetallop Proteinase-1 resulting from increased production of tissue inhibitors of met alloproteinases and -2 by chronic wound fibreblasts.
Changes in cellular motility and cytoskeletal actin in fibroblasts from patients with chronic venous insufficiency and in neonatal fibroblasts in the presence of chronic wound fluid.
TLDR
It appears that CVUWF causes changes in motility and alpha-sma expression in nf-fb as demonstrated in du-fb, and reduced motility in the dermal fb of patients with CVI is demonstrated.
The PHSRN sequence induces extracellular matrix invasion and accelerates wound healing in obese diabetic mice.
TLDR
Analysis of sectioned db/db wounds shows that a single Ac-PHSRN-NH(2) treatment stimulates keratinocyte and fibroblast migration into wounds, enhances fibroplasia and vascularization in the provisional matrix, and stimulates the formation of prominent fibers that may be associated with wound contraction.
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