Madin-Darby canine kidney cells. III. Aldosterone stimulates an apical H+/K+ pump.

@article{Oberleithner1990MadinDarbyCK,
  title={Madin-Darby canine kidney cells. III. Aldosterone stimulates an apical H+/K+ pump.},
  author={Hans Oberleithner and Wieland Steigner and Stefan Silbernagl and Ulrich Vogel and Gerhard Gstraunthaler and Walter Pfaller},
  journal={Pflugers Archiv : European journal of physiology},
  year={1990},
  volume={416 5},
  pages={
          540-7
        }
}
Functionally and morphologically, Madin-Darby canine kidney (MDCK) cells resemble intercalated cells of urinary epithelia. Experiments were performed on domes of confluent MDCK monolayers to test for apical H+ secretion. Apical application of 10(-3) mol/l amiloride or of Na(+)-free solution significantly reduced the limiting pH gradient across the dome epithelium (delta pHd) consistent with inhibition of apical Na+/H+ exchange. Short-circuit current (SCC) measurements disclosed an acetazolamide… CONTINUE READING
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