Madin-Darby canine kidney cells. I. Aldosterone-induced domes and their evaluation as a model system.

@article{Oberleithner1990MadinDarbyCK,
  title={Madin-Darby canine kidney cells. I. Aldosterone-induced domes and their evaluation as a model system.},
  author={Hans Oberleithner and Ulrich Vogel and Ulrich Kersting},
  journal={Pflugers Archiv : European journal of physiology},
  year={1990},
  volume={416 5},
  pages={526-32}
}
Vectorial transport of salt and water in the Madin-Darby canine kidney (MDCK) cell line is indicated by the formation of domes when a monolayer is grown on an impermeable support. We investigated aldosterone-induced dome formation and evaluated the dome as an experimental model. Transepithelial dome resistance was about 80 omega cm2 and constant when dome size exceeded 2.10(-4) cm2. The relative ion conductances (expressed as transference numbers) across the dome epithelium were tNa:tCl:tk = 0… CONTINUE READING