Mad2 binding by phosphorylated kinetochores links error detection and checkpoint action in mitosis

@article{Waters1999Mad2BB,
  title={Mad2 binding by phosphorylated kinetochores links error detection and checkpoint action in mitosis},
  author={Jennifer C Waters and R H Chen and Andrew W. Murray and Gary J Gorbsky and E. D. Salmon and R. Bruce Nicklas},
  journal={Current Biology},
  year={1999},
  volume={9},
  pages={649-652}
}
The spindle checkpoint must detect the presence of unattached or improperly attached kinetochores and must then inhibit progression through the cell cycle until the offending condition is resolved. Detection probably involves attachment-sensitive kinetochore phosphorylation (reviewed in [1,2]). A key player in the checkpoint's response is the Mad2 protein, which prevents activation of the anaphase-promoting complex (APC) by the Cdc20 protein [3-8]. Microinjection of Mad2 antibodies results in… CONTINUE READING