Macromolecular metabolism of a differentiated rat keratinocyte culture system following exposure to sulfur mustard.

@article{Vaughan1988MacromolecularMO,
  title={Macromolecular metabolism of a differentiated rat keratinocyte culture system following exposure to sulfur mustard.},
  author={Frizell L. Vaughan and Syed Zaman and Rosa Maria Scavarelli and Isadore A. Bernstein},
  journal={Journal of toxicology and environmental health},
  year={1988},
  volume={23 4},
  pages={
          507-18
        }
}
A method for producing a stratified, squamous epithelium in vitro by cultivating rat keratinocytes on nylon membranes has been developed in this laboratory. This epidermal-like culture is being used to obtain a better understanding of the mechanism of skin vesication after topical exposure to the sulfur mustard bis(beta-chloroethyl) sulfide (BCES) dissolved in a selected solvent. Radiolabeled macromolecular precursors (thymidine, uridine, and leucine) have been used to study the effect of BCES… CONTINUE READING