Macromolecular diffusion of biological polymers measured by confocal fluorescence recovery after photobleaching.

@article{Gribbon1998MacromolecularDO,
  title={Macromolecular diffusion of biological polymers measured by confocal fluorescence recovery after photobleaching.},
  author={Phillip N Gribbon and Tim E. Hardingham},
  journal={Biophysical journal},
  year={1998},
  volume={75 2},
  pages={1032-9}
}
Fluorescence recovery after photobleaching with an unmodified confocal laser scanning microscope (confocal FRAP) was used to determine the diffusion properties of network forming biological macromolecules such as aggrecan. The technique was validated using fluorescein isothiocyanate (FITC)-labeled dextrans and proteins (molecular mass 4-2000 kDa) at 25 degrees C and with fluorescent microspheres (207 nm diameter) over a temperature range of 5-50 degrees C. Lateral diffusion coefficients (D… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 33 extracted citations

Similar Papers

Loading similar papers…