MYPT1 isoforms expressed in HEK293T cells are differentially phosphorylated after GTPγS treatment

@inproceedings{Lin2016MYPT1IE,
  title={MYPT1 isoforms expressed in HEK293T cells are differentially phosphorylated
after GTPγS treatment},
  author={Simon Lin and Frank V. Brozovich},
  booktitle={Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi},
  year={2016}
}
Agonist stimulation of smooth muscle is known to activate RhoA/Rho kinase signaling, and Rho kinase phosphorylates the myosin targeting subunit (MYPT1) of myosin light chain (MLC) phosphatase at Thr696 and Thr853, which inhibits the activity of MLC phosphatase to produce a Ca2+ independent increase in MLC phosphorylation and force (Ca2+ sensitization). Alternative mRNA splicing produces four MYPT1 isoforms, which differ by the presence or absence of a central insert (CI) and leucine zipper (LZ… CONTINUE READING