MUC1 cytoplasmic tail detection using CT33 polyclonal and CT2 monoclonal antibodies in breast and colorectal tissue.

@article{Croce2006MUC1CT,
  title={MUC1 cytoplasmic tail detection using CT33 polyclonal and CT2 monoclonal antibodies in breast and colorectal tissue.},
  author={Mar{\'i}a Virginia Croce and Marina T. Isla-Larrain and F Remes-Lenicov and Andrea Gladys Colussi and Ezequiel Lacunza and Kyung Chan Kim and Sandra J Gendler and Amada Segal-Eiras},
  journal={Histology and histopathology},
  year={2006},
  volume={21 8},
  pages={849-55}
}
UNLABELLED The immunohistochemical detection (IHC) of MUC1-CT employing a polyclonal antibody (CT33) in relation to CT2 monoclonal antibody (MAb) was analyzed. Western blot (WB) was used to determine the molecular mass of CT. MATERIALS AND METHODS We studied 163 breast and 89 colorectal cancer specimens, 10 breast and 14 colorectal benign conditions, and 12 breast and 20 colorectal normal samples. From each tumor sample, subcellular fractions were obtained and analyzed by SDS-PAGE and WB. A… CONTINUE READING