MTT colorimetric assay detects mitogen responses of spleen but not blood lymphocytes.

@article{Chen1990MTTCA,
  title={MTT colorimetric assay detects mitogen responses of spleen but not blood lymphocytes.},
  author={C. Hai-Peng Chen and Priscilla A. Campbell and Lee S. Newman},
  journal={International archives of allergy and applied immunology},
  year={1990},
  volume={93 2-3},
  pages={249-55}
}
In an effort to simplify methods for assessing mitogen stimulation of blood lymphocytes, the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) colorimetric assay was compared with the conventional tritiated thymidine deoxyriboside (3H-TdR) incorporation assay. Although able to detect mitogen responses by mouse and rat spleen cells, the MTT assay did not reliably measure blood mononuclear cell responses in mouse, rat or man. The 3H-TdR incorporation assay was effective in every… CONTINUE READING