MSL3 coordinates a transcriptional and translational meiotic program in female Drosophila

@article{McCarthy2019MSL3CA,
  title={MSL3 coordinates a transcriptional and translational meiotic program in female Drosophila},
  author={Alicia McCarthy and Kahini Sarkar and Elliot Todd Martin and Maitreyi Upadhyay and Joshua R James and Jennifer M. Lin and Seoyeon Jang and Nathan D Williams and Paolo E. Forni and Michael Buszczak and Prashanth Rangan},
  journal={bioRxiv},
  year={2019}
}
Gamete formation from germline stem cells (GSCs) is essential for sexual reproduction. However, the regulation of GSC differentiation and meiotic entry are incompletely understood. Set2, which deposits H3K36me3 modifications, is required for differentiation of GSCs during Drosophila oogenesis. We discovered that the H3K36me3 reader Male-specific lethal 3 (MSL3) and the histone acetyltransferase complex Ada2a-containing (ATAC) cooperate with Set2 to regulate entry into meiosis in female… Expand
A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis
TLDR
A previously unappreciated TOP-motif in Drosophila responds to reduced ribosome biogenesis to co-regulate the translation of ribosomal proteins and a p53 repressor, thus coupling ribosomopathies to GSC differentiation. Expand
RNA degradation is required for the germ-cell to maternal transition in Drosophila
TLDR
It is shown that a subset of RNAs expressed during the early stages of oogenesis is subjected to regulated degradation during oocyte specification, which helps orchestrate a germ cell-to-maternal transition that gives rise to the maternal contribution to the zygote. Expand
Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis
TLDR
Evidence is found that, although dosage compensation occurs in somatic and pre-meiotic germ cells in Drosophila testis, there might be non-canonical factors involved in the dosage compensation mechanism. Expand
Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis
TLDR
Results suggest that, although DC occurs in somatic and premeiotic germ cells in Drosophila testis, there might be non-canonical factors involved in the dosage compensation, and evidence that the X chromosome is equally transcriptionally active as autosomes in somatics and pre-meiotic cells, and less transcriptionallyactive than autosome in meiotic and post-meiosis cells. Expand
Distinct mechanisms mediate X chromosome dosage compensation in Anopheles and Drosophila
CRISPR knockout of msl-2 and epigenome analyses in Anopheles reveal that X chromosome dosage compensation in mosquitos and Drosophila is achieved by two different molecular mechanisms. SexExpand

References

SHOWING 1-10 OF 298 REFERENCES
The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline
TLDR
YTHDC2 binds multiple transcripts including Ccna2 and other mitotic transcripts, binds specific piRNA precursors, and interacts with RNA granule components, suggesting that proper progression of germ cells through meiosis is licensed by YTH DC2 through post-transcriptional regulation. Expand
Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts
TLDR
The results indicate that proper engagement into meiosis necessitates the specific stabilization of meiotic transcripts, a previously little-appreciated feature in mammals, and propose that the complete induction of the meiotic programme requires both retinoic acid-dependent and -independent mechanisms. Expand
Drosophila Ataxin 2-binding protein 1 marks an intermediate step in the molecular differentiation of female germline cysts
TLDR
It is reported that the early development of germline cysts depends on the Drosophila homolog of the human ataxin 2-binding protein 1 (A2BP1) gene, and biochemical analysis reveals that A2 BP1 promotes the molecular differentiation of ovarian germline Cysts. Expand
Tip60 complex promotes expression of a differentiation factor to regulate germline differentiation in female Drosophila
TLDR
It is found that Nipped-A is required for efficient exit from the gap phase 2 (G2) of cell cycle of the GSC daughter and for expression of a differentiation factor, bgcn, and results indicate that Tip60 complex coordinates cell cycle progression and expression of bgCN to help drive GSC daughters toward a differentiation program. Expand
A discrete transcriptional silencer in the bam gene determines asymmetric division of the Drosophila germline stem cell
TLDR
It is found that bam transcription is active in young germ cells but is repressed specifically in GSCs, and asymmetry of the GSC division can be reduced to identifying the mechanism that selectively activates the silencer element in G SCs. Expand
Stra8 and its inducer, retinoic acid, regulate meiotic initiation in both spermatogenesis and oogenesis in mice
TLDR
It is concluded that, in mice, Stra8 regulates meiotic initiation in both spermatogenesis and oogenesis, and, in both the male and female germ lines, meiosis is initiated through retinoic acid induction of Stra8. Expand
Drosophila dSet2 functions in H3-K36 methylation and is required for development.
TLDR
It is demonstrated that Drosophila d set2 encodes a developmentally essential histone H3 lysine 36 (K36) methyltransferase, and that dSet2 associates with the hyperphosphorylated form of RNA polymerase II (RNAPII). Expand
Drosophila Inducer of MEiosis 4 (IME4) is required for Notch signaling during oogenesis
TLDR
This study reveals the function of IME4 in a metazoan by identifying the phenotypes of hypomorphic alleles of Dm ime4 that allow recovery of viable adults and indicates an evolutionarily conserved function for this enzyme in gametogenesis. Expand
Meioc maintains an extended meiotic prophase I in mice
TLDR
It is shown that, in mice, maintenance of an extended meiotic prophase I requires the gene Meioc, a germ-cell specific factor conserved in most metazoans that promotes a meiotic (as opposed to mitotic) cell cycle program via post-transcriptional control of their target transcripts. Expand
Female Meiosis: Synapsis, Recombination, and Segregation in Drosophila melanogaster
TLDR
Advances in genetic and genomic approaches allow a reconsideration of meiotic phenomena such as interference and the centromere effect, which were previously described only by genetic studies. Expand
...
1
2
3
4
5
...