MS-H: A Novel Proteomic Approach to Isolate and Type the E. coli H Antigen Using Membrane Filtration and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

@inproceedings{Cheng2013MSHAN,
  title={MS-H: A Novel Proteomic Approach to Isolate and Type the E. coli H Antigen Using Membrane Filtration and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)},
  author={Keding Cheng and Michael A. Drebot and Joanne Karen McCrea and Lorea Angel Mary Peterson and D Lee and Stuart J. McCorrister and Richard d. Nickel and Alyssia Gerbasi and Angela Sloan and Debra Janella and Gary H Van Domselaar and Daniel R. Beniac and Todd Booth and Linda Chui and Helen A Tabor and Garrett Westmacott and Marie Naomi Gilmour and Gehua Wang},
  booktitle={PloS one},
  year={2013}
}
Serotyping is the long-standing gold standard method to determine E. coli H antigens; however, this method requires a panel of H-antigen specific antibodies and often culture-based induction of the H-antigen flagellar motility. In this study, a rapid and accurate method to isolate and identify the Escherichia coli (E. coli) H flagellar antigen was developed using membrane filtration and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Flagella were isolated from pure culture, digested… CONTINUE READING

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