MPM-2 antibody-reactive phosphorylations can be created in detergent-extracted cells by kinetochore-bound and soluble kinases.

@article{Renzi1997MPM2AP,
  title={MPM-2 antibody-reactive phosphorylations can be created in detergent-extracted cells by kinetochore-bound and soluble kinases.},
  author={Luigina Renzi and Michael S. Gersch and Michael S. Campbell and Lijun Wu and Stephen A Osmani and Gary J Gorbsky},
  journal={Journal of cell science},
  year={1997},
  volume={110 ( Pt 17)},
  pages={2013-25}
}
The MPM-2 antibody labels mitosis-specific and cell cycle-regulated phosphoproteins. The major phosphoproteins of mitotic chromosomes recognized by the MPM-2 antibody are DNA topoisomerase II (topoII) alpha and beta. In immunofluorescence studies of PtK1 cytoskeletons, prepared by detergent lysis in the presence of potent phosphatase inhibitors, the MPM-2 antibody labels phosphoproteins found at kinetochores, chromosome arms, midbody and spindle poles of mitotic cells. In cells extracted… CONTINUE READING

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