Corpus ID: 32098326

MOLECULAR BIOLOGY AND PHYSIOLOGY Towards Improved Cell Cycle Synchronization and Chromosome Preparation Methods in Cotton

  title={MOLECULAR BIOLOGY AND PHYSIOLOGY Towards Improved Cell Cycle Synchronization and Chromosome Preparation Methods in Cotton},
  author={Randal Halfmann and David M. Stelly and David H Young},
Cotton is an economically important crop that needs more extensive genetic characterization. Highly effective methods for consistent cell cycle manipulation are needed to efficiently produce high quality cytological preparations of chromosomes, and to improve methods of chromosome doubling, both of which impact cotton genomics and breeding. This manuscript reports a procedure for cell cycle synchronization of root tips using hydroxyurea (HU), and the efficacy of several known chemicals for… Expand
Mitotic chromosome doubling of plant tissues in vitro
This review gives an overview of the common methods of chromosome doubling in vitro, the history of the technique, and progress made over the years. Expand
BREEDING AND GENETICS Development of an Improved Method of Mitotic Metaphase Chromosome Preparation Compatible for Fluorescence in situ Hybridization in Cotton
The results show that the combination of a 95-minute N2O pre-treatment, a 55-minute enzymatic degradation, and the “drop method” efficiently produce cotton mitotic metaphase preparations, which will expectedly improve the efficiency and efficacy of GISH, FISH, and single-copy multicolor FISH. Expand
A flow cytometry-based analysis to establish a cell cycle synchronization protocol for Saccharum spp.
The mitotic index, lysis buffer, hydroxyurea(HU) concentration, HU treatment time and recovery time and WPB buffer was superior to other buffers for preparation of sugarcane nuclei suspensions and the CCS protocol defined in this study should accelerate the development of genomic research and cytobiology research in Sugarcane. Expand
Technical Review: Cytogenetic Tools for Studying Mitotic Chromosomes.
In this technical review, different cytogenetic approaches are discussed for the analysis of plant chromosomes, with a focus on mitotic chromosomes. Expand
Development and application of an optimized drop-slide technique for metaphase chromosome spreads in maize
  • Q. Yao, J. Gao, F. Chen, Wenbo Li
  • Medicine, Biology
  • Biotechnic & histochemistry : official publication of the Biological Stain Commission
  • 2019
The optimized method provides a simple, efficient and reproducible procedure for preparing high quality plant chromosome spreads and found no relation between the C-band number and B chromosome. Expand
Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses
The study concludes that the genome size of G. mangostana is between 6.00 and 6.05 pg, which could be due to the occurrence of mutation and aneuploidy in the fruit. Expand
Discovery and characterization of prions in Saccharomyces cerevisiae
It is demonstrated with the newly discovered prion protein, Mot3, that prions accelerate the appearance of new phenotypes in important traits like mating behavior and cell-adhesion, and it is shown that elimination of these prion states has strong phenotypic effects in these strains. Expand


Cell synchronization and isolation of metaphase chromosomes from maize (Zea mays L.) root tips for flow cytometric analysis and sorting.
Levels of hydroxyurea, a DNA synthesis inhibitor, were assessed for their utility in accumulating cells at the G1 phase of the cell cycle, and Chromosome peaks of the observed flow karyotype resembled the predicted flow kARYotype calculated on the basis of maize chromosome size. Expand
Flow Karyotyping and Sorting of Mitotic Chromosomes of Barley (Hordeum vulgare L.)
While it was possible to discriminate and sort only one chromosome from a barley cultivar with standard karyotype, up to three chromosomes could be sorted in translocation lines with morphologically distinct chromosomes. Expand
A high-yield procedure for isolation of metaphase chromosomes from root tips of Vicia faba L.
A new method is described for the isolation of large quantities of Vicia faba metaphase chromosomes and the quality and the quantity of isolated chromosomes, examined microscopically and by flow cytometry, depended on the extent of the fixation. Expand
Cell cycle synchronization in plant root meristems.
A protocol for cell cycle synchronization in root tips of Vicia faba is described, which is based on the use of DNA synthesis inhibitor hydroxyurea, and modifications of the protocol for Pisum sativum, Medicago sativa, Hordeum vulgare, Secale cereale, Triticum aestivum and Zea mays are given. Expand
Preparation and flow cytometric analysis of metaphase chromosomes of tomato
Ch Chromosome suspensions prepared in MgSO4 buffer have the highest frequency of intact chromosomes and the least fluorescent cellular debris and Peaks similar to theoretical univariate flow karyotypes of tomato chromosomes were seen on the observed univariateFlow cytometric analysis. Expand
Antimicrotubule herbicides for in vitro chromosome doubling in Beta vulgaris L. ovule culture
In vitro chromosome doubling during ovule culture of sugar and fodder beets (Beta vulgaris L.) was studied with four anti-microtubule herbicides: amiprophos-methyl (APM), oryzalin, pronamide, andExpand
Integrated karyotyping of sorghum by in situ hybridization of landed BACs.
The results indicate that linkage and physical maps of sorghum allow facile selection of BAC clones according to position and FISH-signal quality, which will enable development of a high-quality molecular cytogenetic map and an integrated genomics system for sorghums, without need of chromosome flow sorting or microdissection. Expand
The effect of trifluralin on the ultrastructure of dividing cells of the root meristem of cotton (Gossypium hirsutum L. "Acala 4-42').
Ultrastructural studies of trifluralin-treated cells in lateral root meristems of cotton revealed that mitotic disruptions were due to the absence of microtubules, and the mitotic process was arrested at the stage that had been reached when the disappearance occurred. Expand
Norflurazon (SAN-9789) reduces abscisic acid levels in cotton seedlings: A glandless isoline is more sensitive than its glanded counterpart
Results indicate that a pathway for abscisic acid biosynthesis in cotton involves carotenoid biosynthesis and that the glanded isoline tolerated approximately 5 to 10 times higher concentrations of norflurazon compared to the glandless isolate. Expand
The biochemistry of compounds with anti-microtubule activity in plant cells.
This paper summarizes the current understanding of the biochemical interactions of colchicine, anti-microtubule herbicides, and taxol with plant tubulin and microtubules. Expand