MO-TMS Derivatives of Human Urinary Steroids for GC and GC-MS Studies

@inproceedings{Thenot1972MOTMSDO,
  title={MO-TMS Derivatives of Human Urinary Steroids for GC and GC-MS Studies},
  author={J.-P. Thenot and Evan C. Horning},
  year={1972}
}
Abstract Sterically hindered 17α-hydroxyl groups in steroids are silylated by trimethylsilylimidazole (TSIM) at 100° C. Decreasing rates of reaction were found for the following structures: 17α, 20β, 21-triol > 17α, 20α-diol > 17α, 20α, 21-triol. When methoxime derivatives are formed as the first step, HCL present in the excess of reagent catalyses the silylation reaction of hydroxyl groups. A simple procedure is described whereby methoxime (MO) derivatives are formed in 15 minutes at 60° C… CONTINUE READING

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