MNase titration reveals differences between nucleosome occupancy and chromatin accessibility.

Abstract

Chromatin accessibility plays a fundamental role in gene regulation. Nucleosome placement, usually measured by quantifying protection of DNA from enzymatic digestion, can regulate accessibility. We introduce a metric that uses micrococcal nuclease (MNase) digestion in a novel manner to measure chromatin accessibility by combining information from several… (More)
DOI: 10.1038/ncomms11485

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Cite this paper

@article{Mieczkowski2016MNaseTR, title={MNase titration reveals differences between nucleosome occupancy and chromatin accessibility.}, author={Jakub Mieczkowski and April Cook and Sarah K. Bowman and Britta Mueller and Burak Han Alver and Sharmistha Kundu and Aim{\'e}e M. Deaton and Jennifer A Urban and Erica Larschan and Peter J. Park and Robert E. Kingston and Michael Y. Tolstorukov}, journal={Nature communications}, year={2016}, volume={7}, pages={11485} }