MHC class I surface expression in embryo-derived cell lines inducible with peptide or interferon

@article{Bikoff1991MHCCI,
  title={MHC class I surface expression in embryo-derived cell lines inducible with peptide or interferon},
  author={Elizabeth K. Bikoff and Leah Jaffe and Randall K. Ribaudo and Gillis R. Otten and Ronald N. Germain and Elizabeth J. Robertson},
  journal={Nature},
  year={1991},
  volume={354},
  pages={235-238}
}
IT has long been recognized that the absence of expression of products of the major histocompatibility complex (MHC) during early development might allow the fetus to escape recognition by maternal lymphocytes. In addition to the MHC class I heavy chain and β2-microglobulin, antigenic peptide is an essential structural component of the class I molecule1–5. Indeed, there is evidence that MHC-linked genes encoding peptide transporter molecules6–10 and possibly components of a proteolytic… Expand
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TLDR
It is shown that in both cell lines the endogenous MHC class I cell surface expression was completely down-regulated, and polymorphic residues in TAP1 can influence the substrate selectivity of the Syrian hamster peptide transporter. Expand
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TLDR
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Considering that EE2H3 was established from primary cultures of mouse embryo cells without immunoselection, and is therefore likely to represent a cell population normally present in post‐implantation‐stage embryos, these findings raise the possibility that expression of class I surface antigens during early development may in part be controlled post‐translationally at the level of MHC class I assembly. Expand
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Gene transfer experiments show that expression of PSF complementary DNA in the human lymphoblastoid cell line mutant 721.174 mutant cells restores normal levels of surface HLA-A2 and -B5, and suggest that at least one of these genes may be required for PSF function. Expand
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It is reported here that culture of the murine lymphoma mutant cell line RMA-S at reduced temperature promotes assembly, and results in a high level of cell surface expression of H-2/β2-microglobulin complexes that do not present endogenous antigens, and are labile at 37 °C. Expand
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