MGE-PCR: a novel approach to the analysis of Toxoplasma gondii strain differentiation using mobile genetic elements.

@article{Terry2001MGEPCRAN,
  title={MGE-PCR: a novel approach to the analysis of Toxoplasma gondii strain differentiation using mobile genetic elements.},
  author={Rebecca S Terry and Judith E. Smith and P Duncanson and Geoff Hide},
  journal={International journal for parasitology},
  year={2001},
  volume={31 2},
  pages={155-61}
}
The position of mobile genetic elements (MGE) within eukaryotic genomes is often highly variable and we have exploited this phenomenon to develop a novel approach to strain differentiation in Toxoplasma gondii. Two PCR based strategies were designed in which specific primers were used to amplify T. gondii MGE's revealing information on element size and positional variation. The first PCR strategy involved the use of a standard two primer PCR while the second strategy used a single specific… CONTINUE READING

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