METHOD FOR THE ASSAY OF ANTIDIURETIC HORMONE IN PLASMA WITH A NOTE ON THE ANTIDIURETIC TITER OF HUMAN PLASMA.

@article{Yoshida1963METHODFT,
  title={METHOD FOR THE ASSAY OF ANTIDIURETIC HORMONE IN PLASMA WITH A NOTE ON THE ANTIDIURETIC TITER OF HUMAN PLASMA.},
  author={S Yoshida and Kenji Motohashi and Hiroshi Ibayashi and Shigeo Okinaka},
  journal={The Journal of laboratory and clinical medicine},
  year={1963},
  volume={62},
  pages={
          279-85
        }
}
Abstract A method for the extraction and purification of antidiuretic hormone (ADH) from human plasma is described. Share's original procedure involves the trichloroacetic acid extraction of ADH from plasma, ether wash, and purification of the extract with XE-64 resin column chromatography followed by the ADH assay in water-loaded, ethanol-anesthetized rats with changes in conductivity of the urine as the index of response. This was modified by the use of 50 per cent acetic acid instead of… 
Recovery of ADH activity in the urine of goats under normal and stimulated conditions.
TLDR
The hydrated goat was used for bioassays of antidiuretic hormone (ADH) activity recovered by resin column separation from the urine of other animals of the same species, and it was possible to determine to the nearest 0.5 of a mU the ADH activity of unknown samples, provided they contained between 0-5 mU of ADH.
Radioimmunoassay of arginine vasopressin in human plasma and urine, a resin microcolumn method.
TLDR
A new method was developed for the estimation of arginine vasopressin (AVP) in plasma and urine using Ion-exchange resin, CG-50, H+ form, packed in a small column to remove the interfering substances and to concentrate the AVP in the sample.
Development and clinical application of a new method for the radioimmunoassay of arginine vasopressin in human plasma.
TLDR
The radioimmunoassay method provides a useful way of assessing AVP function in man and, when used appropriately, approximate the anticipated levels and vary in accordance with physiologic expections.
Assays for Vasopressin
Metabolism of antidiuretic hormones.
  • H. Lauson
  • Medicine, Biology
    The American journal of medicine
  • 1967
Increased plasma arginine vasopressin in clinical adrenocortical insufficeincy and its inhibition by glucosteroids.
TLDR
It is suggested that hypersecretion of AVP may play an important role in the abnormal water metabolism of adrenocortical insufficiency and that the glucosteroids promote normal water diuresis by inhibiting the secretion of A VP from the neurohypophysis.
The Assay of Neurohypophysial Hormones in Blood and Other Body Fluids
TLDR
This section will be mainly orientated around the detection and measurement of oxytocin and vasopressin at low concentrations, such as may occur naturally in blood or urine, when in general there is no practical limit to the amount of active substance available.
Resin column chromatography has been used frequently for the radioimmunoas say of urinary AVP ( Miller and Moses
TLDR
A new method was developed for the estimation of arginine vasopressin (AVP) in plasma and urine and samples were extracted by a microcolumn of resin and assayed radioimmunologically using a highly sensitive antiserum to AVP.
Distribution, blood transport, and degradation of antidiuretic hormone in man.
TLDR
It is concluded that in man antidiuretic hormone circulates as a free (non-protein bound) peptide, diffuses readily into the extracellular fluid space, and is metabolized within minutes.
Urinary excretion of antidiuretic hormone under various hydration states in man.
TLDR
Two types of daily urinary excretion patterns ofADH were found in patients with SIADH, and either case showed no suppressibility of ADH in the face of an expansion of body fluid with hypo-osmolality of the plasma.
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