MEMBRANE IONIC CURRENT PHOTOMODIFICATION BY ROSE BENGAL AND MENADIONE: ROLE OF SINGLET OXYGEN

@article{Arriaga1994MEMBRANEIC,
  title={MEMBRANE IONIC CURRENT PHOTOMODIFICATION BY ROSE BENGAL AND MENADIONE: ROLE OF SINGLET OXYGEN},
  author={Edgar Arriaga and Andrey Frolov and Merrill Tarr and Dennis Paul Valenzeno},
  journal={Photochemistry and Photobiology},
  year={1994},
  volume={59}
}
Abstract: Photosensitized modification of ionic leak current and potassium current was studied in frog cardiac atrial cells using whole cell patch clamp techniques. Rose bengal (RB) and menadione (MQ) were used as photosensitizers. Separate photophysical studies of the photosensitizers in deuterium oxide solution demonstrated that MQ did not produce singlet oxygen as evidenced by the lack of luminescence at 1270 nm, whereas RB was an efficient singlet oxygen generator. Both photosensitizers… 
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References

SHOWING 1-10 OF 29 REFERENCES
MEMBRANE POTENTIAL CAN INFLUENCE THE RATE OF MEMBRANE PHOTOMODIFICATION
TLDR
It is demonstrated that the rate of photomodification of potassium channels in frog atrial cells is voltage dependent, and similar effects on potassium current were obtained using either a positively charged or negatively charged sensitizer.
MEMBRANE PHOTOMODIFICATION OF CARDIAC MYOCYTES: POTASSIUM AND LEAKAGE CURRENTS
TLDR
The stabilization of Ik following illumination suggests that the photomodification of the potassium channel does not involve long lasting (minutes) radical chain reactions and that this photommodification is not repaired in the course of a few minutes.
CELL MEMBRANE PHOTOMODIFICATION: RELATIVE EFFECTIVENESS OF HALOGENATED FLUORESCEINS FOR PHOTOHEMOLYSIS
TLDR
The results suggest that the influence of halogen substitution on sensitizing potency is exerted by four factors; distribution of sensitizer into a low polarity region of the cell membrane, absorption efficiency in a lowPolarity region, triplet quantum yield, and photobleach rate.
Modification of cardiac action potential by photosensitizer-generated reactive oxygen.
PHOTOBLEACHING OF MEROCYANINE 540: INVOLVEMENT OF SINGLET MOLECULAR OXYGEN
TLDR
The rate of self‐sensitized photobleaching was enhanced in D2O and inhibited by both azide and histidine, consistent with 1O2 intermediacy (Type II chemistry), and a rate constant of 107M‐1 s‐1 was determined for the chemical quenching of 1 O2 by MC540.
PHOTOOXIDATION OF CELL MEMBRANES USING EOSIN DERIVATIVES THAT LOCATE IN LIPID OR PROTEIN TO STUDY THE ROLE OF DIFFUSIBLE INTERMEDIATES
  • J. Pooler
  • Chemistry
    Photochemistry and photobiology
  • 1989
TLDR
The membrane photomodification experiments suggest that both sensitizers act via intermediates, possibly singlet oxygen, that reach membrane targets through a diffusion path somewhat shielded from agents in the reaction medium, the access being particularly restricted with EYMA.
Photosensitization: Reaction Pathways
TLDR
Living organisms contain many enzymic and nonenzymic antioxidant mechanisms to protect against reactive oxygen species and the inability to control the latter has been defined as oxidative stress.
PHOTOSENSITIZATION OF PYRIMIDINES BY 2‐METHYLNAPHTHOQUINONE IN WATER: A LASER FLASH PHOTOLYSIS STUDY
TLDR
2‐Methylnaphthoquinone has been excited in water with a 20 ns laser flash at 353 nm and the resultant transient species have been observed optically and the interaction of 3MQ with pyrimidines involves charge transfer to give the pyrimidine cation radical and the MQ : anion radical.
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