MEGA (Multiple Essential Genes Assembling) deletion and replacement method for genome reduction in Escherichia coli.


Top-down reduction of the bacterial genome to construct desired chassis cells is important for synthetic biology. However, the current progress in the field of genome reduction is greatly hindered by indispensable life-essential genes that are interspersed throughout the chromosomal loci. Here, we described a new method designated as "MEGA (Multiple… (More)
DOI: 10.1021/sb500324p


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