MDM2 inhibits p300-mediated p53 acetylation and activation by forming a ternary complex with the two proteins.

@article{Kobet2000MDM2IP,
  title={MDM2 inhibits p300-mediated p53 acetylation and activation by forming a ternary complex with the two proteins.},
  author={E Kobet and Xianlu Zeng and Yuantee Zhu and David Keller and Huiling Lu},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 23},
  pages={12547-52}
}
p300 acetylates and activates the tumor suppressor p53 after DNA damage. Here, we show that MDM2, a negative-feedback regulator of p53, inhibited p300-mediated p53 acetylation by complexing with these two proteins. First, we purified a p300-MDM2-p53 protein complex from HeLa nuclear extracts, which was inactive in p53 acetylation, but active in histone acetylation. Also, wild-type, but not N-terminally deleted, MDM2 inhibited p53 acetylation by p300 in vitro and in vivo. This inhibition was… CONTINUE READING

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