MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis

@article{Samora2011MAP4AC,
  title={MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis},
  author={Catarina P. Samora and Binyam Mogessie and Leslie C. Conway and Jennifer L. Ross and Anne Straube and Andrew D. McAinsh},
  journal={Nature Cell Biology},
  year={2011},
  volume={13},
  pages={1040-1050}
}
Correct positioning of the mitotic spindle is critical to establish the correct cell-division plane. Spindle positioning involves capture of astral microtubules and generation of pushing/pulling forces at the cell cortex. Here we show that the tau-related protein MAP4 and the microtubule rescue factor CLASP1 are essential for maintaining spindle position and the correct cell-division axis in human cells. We propose that CLASP1 is required to correctly capture astral microtubules, whereas MAP4… 

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References

SHOWING 1-10 OF 50 REFERENCES

Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle

These results reveal crucial roles for EB1 in mitosis, which is postulate involves its ability to promote the growth and interactions of microtubules within the central spindle and at the cell cortex.

Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function.

It is shown that ClASP2 localization at kinetochores, centrosomes, and spindle throughout mitosis is remarkably similar to CLASP1, both showing fast microtubule-independent turnover rates.

Heterotrimeric G protein signaling functions with dynein to promote spindle positioning in C. elegans

It is found that the depletion of dyrb-1 enhances the spindle positioning defect of weak loss of function alleles of two regulators of G protein signaling, LIN-5 and GPR-1/2, and that DYRB-1 physically associates with these two proteins.

VHL loss causes spindle misorientation and chromosome instability

The von Hippel-Lindau (VHL) tumour suppressor protein, pVHL, which is inactivated in hereditary and sporadic forms of renal cell carcinoma, localizes to the mitotic spindle in mammalian cells and its functional inactivation provokes spindle misorientation, spindle checkpoint weakening and chromosomal instability.

The spindle-assembly checkpoint in space and time

Recent molecular analyses have begun to shed light on the complex interaction of the checkpoint proteins with kinetochores — structures that mediate the binding of spindle microtubules to chromosomes in mitosis.

Disruption of mitotic spindle orientation in a yeast dynein mutant.

The results suggest that, in yeast, a dynein microtubule motor protein has a nonessential role in spindle assembly and chromosome movement but is involved in establishing the proper spindle orientation during cell division.