MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR

@article{Fushan2009MALSAE,
  title={MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR},
  author={Alexey A. Fushan and Dennis Drayna},
  journal={BMC Biotechnology},
  year={2009},
  volume={9},
  pages={83 - 83}
}
BACKGROUND Multiple approaches for the site-directed mutagenesis (SDM) have been developed. However, only several of them are designed for simultaneous introduction of multiple nucleotide alterations, and these are time consuming. In addition, many of the existing multiple SDM methods have technical limitations associated with type and number of mutations that can be introduced, or are technically demanding and require special chemical reagents. RESULTS In this study we developed a quick and… CONTINUE READING

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