MALDI tissue imaging of ocular lens alpha-crystallin.

  title={MALDI tissue imaging of ocular lens alpha-crystallin.},
  author={Jun Han and Kevin L. Schey},
  journal={Investigative ophthalmology & visual science},
  volume={47 7},
PURPOSE To apply MALDI (matrix-assisted laser desorption ionization) tissue imaging methods to obtaining a profile of the distribution of the lens alpha-crystallins and their modified forms in calf and mature bovine lenses. METHODS Frozen bovine lenses were cut equatorially at -12 degrees C to -20 degrees C into 10- to 40-microm sections depending on lens age. Tissue sections were mounted onto MALDI sample plates by ethanol soft-landing to maintain tissue integrity. A two-layered matrix… CONTINUE READING


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