MALDI mass sequencing and characterization of filarialglutathione-S-transferase.

  title={MALDI mass sequencing and characterization of filarialglutathione-S-transferase.},
  author={Sarika Gupta and Anchal Singh and Marshleen Yadav and Kalyan Singh and Sushma Rathaur},
  journal={Biochemical and biophysical research communications},
  volume={356 2},
Glutathione-S-transferase has been detected in the somatic extract and excretory-secretory products of different life stages of Setaria cervi, a bovine filarial parasite. The enzyme was subjected to MALDI-TOF followed by mass spectrometry and the nearest match found was Pleuronectes platessa GST. Molecular mass of the purified enzyme was approximately 26 kDa as determined by SDS-PAGE and MALDI-TOF. Setaria cervi GST exhibited high activity towards 1-chloro-2,4-dinitrobenzene and ethacrynic acid… CONTINUE READING