MALDI-TOF mass spectrometry methods for evaluation of in vitro aminoacyl tRNA production.

@article{Petersson2002MALDITOFMS,
  title={MALDI-TOF mass spectrometry methods for evaluation of in vitro aminoacyl tRNA production.},
  author={E James Petersson and Mona Shahgholi and Henry A. Lester and Dennis A Dougherty},
  journal={RNA},
  year={2002},
  volume={8 4},
  pages={542-7}
}
Unnatural amino acid mutagenesis requires the in vitro production of aminoacyl tRNAs. Bacteriophage T4 RNA ligase is used to ligate a-amino-protected dCA amino acids to 74mer tRNA. Previously, there has been no facile method for evaluating the efficiency of this reaction prior to using the tRNA in translation. We report a novel use of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry in monitoring the formation of aminoacyl 76mer tRNA. This method is more efficient and… CONTINUE READING
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