MALDI-TOF mass spectrometric typing of single nucleotide polymorphisms with mass-tagged ddNTPs.

@article{Fei1998MALDITOFMS,
  title={MALDI-TOF mass spectrometric typing of single nucleotide polymorphisms with mass-tagged ddNTPs.},
  author={Zhizhong Fei and Tsunehiko Ono and Lloyd M. Smith},
  journal={Nucleic acids research},
  year={1998},
  volume={26 11},
  pages={2827-8}
}
A matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based method has recently been reported for the typing of single nucleotide polymorphisms using single nucleotide primer extension. This method is limited in some cases by the resolution of the mass determination, as the mass difference between nucleotides can be as little as 9 Da (the difference between A and T). A variation of this method is described here in which a mass-tagged dideoxynucleotide is employed in the… CONTINUE READING