MALDI-TOF mass spectrometric detection of multiplex single base extended primers. A study of 17 y-chromosome single-nucleotide polymorphisms.

@article{MengelJrgensen2004MALDITOFMS,
  title={MALDI-TOF mass spectrometric detection of multiplex single base extended primers. A study of 17 y-chromosome single-nucleotide polymorphisms.},
  author={J Mengel-J\orgensen and Juan Jose Sanchez and Claus B\orsting and Finn Kirpekar and Niels Morling},
  journal={Analytical chemistry},
  year={2004},
  volume={76 20},
  pages={6039-45}
}
One of the most promising techniques for typing of multiple single-nucleotide polymorphism (SNP) is detection of single base extension primers (SBE) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We present a new MALDI-TOF MS protocol for typing of multiple SNPs in a single reaction. Biotin-labeled ddNTPs were used in the SBE reaction and solid phase-bound monomeric avidin was used as capturing/purification scheme allowing the exclusive release… CONTINUE READING

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