MALDI-TOF MS and CE-LIF Fingerprinting of plant cell wall polysaccharide digests as a screening tool for arabidopsis cell wall mutants.

@article{Westphal2010MALDITOFMA,
  title={MALDI-TOF MS and CE-LIF Fingerprinting of plant cell wall polysaccharide digests as a screening tool for arabidopsis cell wall mutants.},
  author={Yvonne Westphal and H A Schols and Alphons G. J. Voragen and Harry Gruppen},
  journal={Journal of agricultural and food chemistry},
  year={2010},
  volume={58 8},
  pages={4644-52}
}
Cell wall materials derived from leaves and hypocotyls of Arabidopsis mutant and wild type plants have been incubated with a mixture of pure and well-defined pectinases, hemicellulases, and cellulases. The resulting oligosaccharides have been subjected to MALDI-TOF MS and CE-LIF analysis. MALDI-TOF MS analysis provided a fast overview of all oligosaccharides released, whereas CE-LIF-measurements enabled separation and characterization of many oligosaccharides under investigation. Both methods… CONTINUE READING

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