MALDI MS peptide mapping performance by in-gel digestion on a probe with prestructured sample supports.

@article{Klen2004MALDIMP,
  title={MALDI MS peptide mapping performance by in-gel digestion on a probe with prestructured sample supports.},
  author={Tina Guldberg Klen\o and Christian Andreasen and Helle \Orsted Kjeldal and Lise R\onnedal Leonardsen and Thomas Nylandsted Krogh and Per Franklin Nielsen and Marianne S\orensen and Ole N\orregaard Jensen},
  journal={Analytical chemistry},
  year={2004},
  volume={76 13},
  pages={3576-83}
}
Matrix-assisted laser desorption/ionization (tandem) mass spectrometry (MALDI MS) is widely used in protein chemistry and proteomics research for the identification and characterization of proteins isolated by polyacrylamide gel electrophoresis. In an effort to minimize sample handling and increase sample throughput, we have developed a novel in-gel digestion protocol where sample preparation is performed directly on a MALDI probe with prestructured sample support. The protocol consists of few… CONTINUE READING