Métodos diagnósticos em tuberculose.

  title={M{\'e}todos diagn{\'o}sticos em tuberculose.},
  author={Jo{\~a}o Bento and Anabela Santos Silva and Filomena Rodrigues and Raquel Duarte},
  journal={Acta M{\'e}dica Portuguesa},
Early diagnosis and treatment are critical to minimize the spread and to reduce morbidity and mortality from tuberculosis. Clinical diagnosis is most time difficult resulting from non-specific and frequently indolent symptoms. Radiological presentation may be very diverse. Currently TB diagnosis still depends on microbiological exams which require a very careful and quick specimen handling. A positive acid-fast bacilli smear makes a rapid presumptive diagnosis. Nevertheless the gold-standard… 

Tuberculose miliar no século XXI: a propósito de um caso clínico

Even in this era of advanced medical technology, tuberculosis is still a diagnostic challenge, especially when the presentation is atypical and extra-pulmonary, and a high index of suspicion by the physician is required because prompt institution of adequate treatment is decisive for final outcome.


The results suggest that the RT-qPCR is a tool that can be used in clinical and therapeutic monitoring as an indicator of bacterial resistance and indicator of the period of transmissibility of M. tuberculosis in patients with pulmonary TB undergoing treatment.

Avaliação da atividade da adenosina deaminase no derrame pleural tuberculoso

The determination of ADA activity is a precise procedure with good sensitivity and can be suggested as an important marker of tuberculosis PE, which could aid in the differential diagnosis of the disease.

The Prevalence of Active Tuberculosis Among Patients With Fibrotic Lesion in Chest CT-Scan

Fibrocalcified lesions should be evaluated for detecting M. tuberculosis particularly in the endemic regions, according to the observations.

Delays in tuberculosis suspicion and diagnosis and related factors.

The study revealed the existence of gaps in TB control activities related to the patients and the organization of the health-care services and showed the importance of taking into account the spatial dependence of the phenomena analyzed.

Tuberculosis in Hematopoietic Stem Cell Transplant Recipients

There is no consensus regarding the screening with tuberculin skin test or QuantiFERON-TB gold, primary prophylaxis for latent TB, and whether the epidemiologic query should be emphasized in developing countries with high prevalence of TB.

Educational intervention for collecting sputum for tuberculosis: a quasi-experimental study 1

It was shown that after the educational intervention, it was observed sputum samples with better quality, with satisfactory aspect and volume for the effectiveness of the bacilloscopic examination.

Nursing outcome "Severity of infection": conceptual definitions for indicators related to respiratory problems.

This study contributed to understand the terms used in the nursing outcome Infection Severity, in order to improve and facilitate the use of the NOC, as it enhances the conceptual clarity of the selected indicators with a view to producing better scientific evidence.

Metalo-proteases de Mycobacterium tuberculosis : características moleculares e funcionais desempenham papel na biologia da interface patógeno-hospedeiro

The World Health Organization (WHO) confirms that there has been a significant increase in the number of cases of tuberculosis around the world in the past 10 years, mainly connected with the problem of infectious disease of the immune system.

Analytical validation of an in‐house method for adenosine deaminase determination

The adenosine deaminase (ADA) enzyme is a marker of inflammatory processes whose activity can be measured through a colorimetric method developed as an in‐house assay. This validation can reduce



Diagnostic tools in tuberculous pleurisy: a direct comparative study

A combination of pleural fluid adenosine deaminase, differential cell count and closed needle biopsy has a high diagnostic accuracy in undiagnosed exudative pleural effusions in areas with high incidences of tuberculosis and might substitute medical thoracoscopy at considerably lower expense in resourcepoor countries.

Novel tests for diagnosing tuberculous pleural effusion: what works and what does not?

An overview of the performance of new diagnostic tests, including markers of specific and nonspecific immune response, nucleic acid amplification and detection, and predictive models based on combinations of markers for pleural tuberculosis are provided.

Cerebrospinal fluid adenosine deaminase activity: A complimentary tool in the early diagnosis of tuberculous meningitis

It is demonstrated that adenosine deaminase activity in the CSF of TBM patients, using a cut-off value 11.39 U/L/min, can be useful for the early differential diagnosis of T BM.

Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta-analysis

The results suggest that commercial NAA tests may have a potential role in confirming (ruling in) tuberculous pleuritis, however, these tests have low and variable sensitivity and, therefore, may not be useful in excluding (ruled out) the disease.

Commercial Nucleic-Acid Amplification Tests for Diagnosis of Pulmonary Tuberculosis in Respiratory Specimens: Meta-Analysis and Meta-Regression

Commercial NAATs alone cannot be recommended to replace conventional tests for diagnosing pulmonary TB and improvements in diagnostic accuracy, particularly sensitivity, need to be made in order for this expensive technology to be worthwhile and beneficial in low-resource countries.

Novel Technique of Quantitative Nested Real-Time PCR Assay for Mycobacterium tuberculosis DNA

Compared to the conventional PCR assay, the QNRT-PCR assay can be considered a more useful and advanced technique for the rapid and accurate diagnosis of TBM.

Novel Wide-Range Quantitative Nested Real-Time PCR Assay for Mycobacterium tuberculosis DNA: Clinical Application for Diagnosis of Tuberculous Meningitis

The WR-QNRT-PCR assay is considered to be a useful and advanced assay technique for assessing the clinical treatment course of TBM.

Biomarkers for Clinical and Incipient Tuberculosis: Performance in a TB-Endemic Country

These biomarkers provide high sensitivity and specificity for TB diagnosis in a TB endemic setting and are not compromised by concurrent HIV infection, site of TB and absence of pulmonary manifestations in HIV+TB+ patients.

Reducing the number of sputum samples examined and thresholds for positivity: an opportunity to optimise smear microscopy.

Reducing the number of specimens and the positivity threshold to define a positive case increased the sensitivity of microscopy and reduced laboratory workload.

Supervised and induced sputum among patients with smear-negative pulmonary tuberculosis

It may be practical to collect a sample of unsupervised, supervised and induced sputum for smear-negative patients with extent of disease less than the right upper lobe, especially when respiratory symptoms are absent.