Lysyl residues of cardiac myosin accessible to labeling with a fluorescent reagent, N-methyl-2-anilino-6-naphthalenesulfonyl chloride.

@article{Hiratsuka1980LysylRO,
  title={Lysyl residues of cardiac myosin accessible to labeling with a fluorescent reagent, N-methyl-2-anilino-6-naphthalenesulfonyl chloride.},
  author={Toshiaki Hiratsuka and K Uchida},
  journal={Journal of biochemistry},
  year={1980},
  volume={88 5},
  pages={
          1437-48
        }
}
Two mol of N-methyl-2-anilino-6-naphthalenesulfonyl (Mns) groups was preferentially incorporated into pig cardiac myosin in the absence of divalent metal ions, 1 mol rapidly and 1 mol slowly. In the presence of divalent metal ions. 1 mol was rapidly incorporated but subsequent incorporation was strongly suppressed. No substantial effect of incorporation of Mns groups in the presence or absence of divalent metal ions on the Ca2+- and K+-ATPase activities of myosin was found. However, the… 
3 Citations
Conformational changes of tarantula (Eurypelma californicum) haemocyanin detected with a fluorescent probe, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole.
TLDR
The sharp shift of the maxima of the emission wavelengths during oxygenation indicates that the four copies of subunits d synchronously take part in the conformational switch of the tarantula haemocyanin.
Fluorescence resonance energy transfer measurements of distances in actin and myosin. A critical evaluation
TLDR
The reliability and consistency of intra- and inter-molecular distances measured between the fluorescent probes attached to specific sites on these proteins are assessed and the contribution of FRET determinations to the current debate on the molecular mechanism of contraction is discussed.