Lysyl hydroxylase. Further purification and characterization of the enzyme from chick embryos and chick embryo cartilage.

@article{Ryhnen1976LysylHF,
  title={Lysyl hydroxylase. Further purification and characterization of the enzyme from chick embryos and chick embryo cartilage.},
  author={Lasse Ryh{\"a}nen},
  journal={Biochimica et biophysica acta},
  year={1976},
  volume={438 1},
  pages={71-89}
}
A purification of up to 4000-fold is reported for lysyl hydroxylase (EC 1.14.11.4) from extract of chick-embryo homogenate and one of about 300-fold from extract of chick-embryo cartilage. Multiple forms of the enzyme were observed during purification from whole chick embryos. In gel filtration the elution positions of the two main forms corresponded to average molecular weights of about 580000 and 220000. These two forms could also be clearly separated in hydroxyapatite chromatography. In… CONTINUE READING