Lysosomal enzymes in bovine mammary leukocytes during the nonlactating period.

@article{Hurley1987LysosomalEI,
  title={Lysosomal enzymes in bovine mammary leukocytes during the nonlactating period.},
  author={Walter L. Hurley and Tomas McKee and D R Cue},
  journal={Veterinary immunology and immunopathology},
  year={1987},
  volume={16 1-2},
  pages={
          95-105
        }
}
Leukocytes from mammary secretions in dairy cows were collected during the nonlactating and postpartum periods. Differential cell counts, viability and activity of peroxidase, N-acetyl-beta-D-glucosaminidase (NAGase, beta-glucuronidase and alpha-mannosidase in cells were determined. Cell viability (trypan blue exclusion) was 75-80% during most of the nonlactating period, but declined to 45-50% by parturition. Polymorphonuclear neutrophils (PMN) predominated during the first week of involution… CONTINUE READING