LysoTracker is a marker of differentiated alveolar type II cells

@inproceedings{Velden2013LysoTrackerIA,
  title={LysoTracker is a marker of differentiated alveolar type II cells},
  author={Joanne L Van der Velden and Ivan Bertoncello and Jonathan L. McQualter},
  booktitle={Respiratory research},
  year={2013}
}
BACKGROUND LysoTracker Green DND-26 is a fluorescent dye that stains acidic compartments in live cells and has been shown to selectively accumulate in lamellar bodies in alveolar type II (AT2) cells in the lung. The aim of this study was to determine whether the accumulation of LysoTracker in lamellar bodies can be used to isolate viable AT2 cells by flow cytometry and track their differentiation in live-cell culture by microscopy. METHODS Mouse lung cells were sorted on the basis of CD45(neg… CONTINUE READING

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