Enzyme preparations of Staphylococcus aureus were examined for their ability to solubilize (32)P-labeled cell walls of the parent organism. Enzymatic activity was observed in the growth medium, in soluble fractions, and associated with native cell walls. Enzyme associated with isolated cell walls could be inactivated with formaldehyde without reducing the… (More)
FIG. 4. Dependence of cell-wall lytic activity of the AFZ enzyme fraction on pH. Assay conditions were as indicated in the standard assay, with the exception that formate (A); acetate (0); phosphate (a) and N-tris(hydroxymethyl) methyl-2-amino-ethanesulfonic acid (A) buffers were used as indicated. AllpH values are the measuredfinalpH of the reaction mixture.