Lysine Uptake by Cloned hCAT-2B: Comparison with hCAT-1 and with Trophoblast Surface Membranes

  title={Lysine Uptake by Cloned hCAT-2B: Comparison with hCAT-1 and with Trophoblast Surface Membranes 
  author={T. Furesz and E. Heath-Monnig and S. G. Kamath and C. Smith},
  journal={The Journal of Membrane Biology},
To study the cationic amino-acid transporter hCAT-2B of human placenta, total RNA was harvested from primary cultured trophoblast and from the BeWo choriocarcinoma cell line (b30 clone) and used for reverse transcription (RT) and polymerase chain reaction (PCR). Primers based on published sequences identified expression of mRNA for hCAT-2B. RT-PCR yielded a 2.06 kb hCAT-2B cDNA, which was cloned. hCAT-2B cRNA injection into Xenopus laevis oocytes stimulated saturable lysine uptake (Km ~ 125 mM… Expand
L-Arginine transport across the basal plasma membrane of the syncytiotrophoblast of the human placenta from normal and preeclamptic pregnancies.
Data suggest increased activity of the BM system y(+) cationic amino acid transporter in preeclampsia is reflected in vivo, and a similar increase in transporter activity could alter the delivery of L-arginine to syncytiotrophoblast eNOS. Expand
Isoforms of amino acid transporters in placental syncytiotrophoblast: plasma membrane localization and potential role in maternal/fetal transport.
This review of placental amino acid transporter isoforms first provides an overview of their properties and preliminary plasma membrane localization, and presents a review of current knowledge of plasma membrane protein localization as derived from investigations with a widely used epithelial model cell line. Expand
Placental amino acid transport systems and fetal growth restriction--a workshop report.
The FGRplacenta has decreased total villous surface area, indicating thatmorphometric changes in vascularization may contribute to theoverall reduction in placental diffusional transport capacity, and both decreased surface area for diffusional exchange forcompounds such as oxygen and reductions in the number ofspecific nutrient transporters and their activities may contrib-ute to a global reduction in nutrient transport in FGRpregnancies. Expand
Fetoplacental transport and utilization of amino acids in IUGR--a review.
Observations in the placental insufficiency model of IUGR indicate that the combination of decreased fetoplacental amino acid uptake and disrupted insulin/IGF signaling in liver and muscle account for decreased fetal growth in IUBR. Expand
Chapter 67 – The Significance of ABC Transporters in Human Placenta for the Exposure of Fetus to Xenobiotics
Human placenta expresses a variety of ATP-binding cassette (ABC) transporters many of which have been shown to be not only protective against fetal exposure but also important for physiologicalExpand
The significance of ABC transporters in human placenta for the exposure of the fetus to xenobiotics
Estimating the contribution of an ABC transporter for fetal exposure localization has to be taken into account in addition to the level of expression, functional status and substrate specificity, because interference in the function of transporters may significantly increase the fetal exposure to xenobiotics or drugs. Expand
Evolution of placental function in mammals: the molecular basis of gas and nutrient transfer, hormone secretion, and immune responses.
  • A. Carter
  • Biology, Medicine
  • Physiological reviews
  • 2012
Coevolution in the primate lineage of killer immunoglobulin-like receptors and human leukocyte antigens can be linked to the deep invasion of the uterus by trophoblast that is a characteristic feature of human placentation. Expand
CHAPTER 52 – Placental Transfer
The placenta is the organ in which the two microcirculations, maternal and fetal, are opposed and across which most, but not all transfer takes place. For any permeant to cross the placenta, it hasExpand