Lysine Uptake by Cloned hCAT-2B: Comparison with hCAT-1 and with Trophoblast Surface Membranes

@article{Furesz2002LysineUB,
  title={Lysine Uptake by Cloned hCAT-2B: Comparison with hCAT-1 and with Trophoblast Surface Membranes 
},
  author={T. Furesz and E. Heath-Monnig and S. G. Kamath and C. Smith},
  journal={The Journal of Membrane Biology},
  year={2002},
  volume={189},
  pages={27-33}
}
To study the cationic amino-acid transporter hCAT-2B of human placenta, total RNA was harvested from primary cultured trophoblast and from the BeWo choriocarcinoma cell line (b30 clone) and used for reverse transcription (RT) and polymerase chain reaction (PCR). Primers based on published sequences identified expression of mRNA for hCAT-2B. RT-PCR yielded a 2.06 kb hCAT-2B cDNA, which was cloned. hCAT-2B cRNA injection into Xenopus laevis oocytes stimulated saturable lysine uptake (Km ~ 125 mM… Expand
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