Lyophilization-induced reversible changes in the secondary structure of proteins.

@article{Griebenow1995LyophilizationinducedRC,
  title={Lyophilization-induced reversible changes in the secondary structure of proteins.},
  author={Kai Griebenow and Alexander M. Klibanov},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1995},
  volume={92 24},
  pages={
          10969-76
        }
}
Changes in the secondary structure of some dozen different proteins upon lyophilization of their aqueous solutions have been investigated by means of Fourier-transform infrared spectroscopy in the amide III band region. Dehydration markedly (but reversibly) alters the secondary structure of all the proteins studied, as revealed by both the quantitative analysis of the second derivative spectra and the Gaussian curve fitting of the original infrared spectra. Lyophilization substantially… CONTINUE READING

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