Lymphokines, including interleukin-2, alter gonadotropin-stimulated progesterone production and proliferation of human granulosa-luteal cells in vitro.

  title={Lymphokines, including interleukin-2, alter gonadotropin-stimulated progesterone production and proliferation of human granulosa-luteal cells in vitro.},
  author={L J Wang and Sarah A. Robertson and Robert F. Seamark and Robert J. Norman},
  journal={The Journal of clinical endocrinology and metabolism},
  volume={72 4},
The effects of human interleukin-1 (IL-1) and IL-2 on human granulosa-luteal cell progesterone production were examined with or without hCG stimulation in vitro. Human granulosa-luteal cells were recovered from follicular fluid obtained from women undergoing in vitro fertilization procedures and cultured for up to 7 days before supernatant progesterone level measurement. Lymphokine-rich conditioned medium was prepared from mitogen-stimulated human peripheral blood leukocytes (HPL-CM). The… 

Cellular composition of primary cultures of human granulosa-lutein cells and the effect of cytokines on cell proliferation.

Any observed effects from the addition of cytokines in this system may be due to indirect effects of cytokine-activated leukocytes on granulosa-lutein cells.

Granulocyte-macrophage colony-stimulating factor: presence in human follicular fluid, protein secretion and mRNA expression by ovarian cells.

Results show that GM-CSF is expressed and secreted by cells within the human ovary, and, together with the finding of expression of mRNA for GM- CSF receptor, suggest a role for GM -CSF in the local regulation of ovarian events.

Interleukin-2 affects steroidogenesis and numbers of bovine ovarian granulosa cells but not thecal cellsin vitro

It appears that granulosa cells are more sensitive to IL-2 than are thecal cells, as they were studied independently under serum-free conditions and media enriched with 10% fetal calf serum.

Interleukin-1-mediated stimulation of prostaglandin E production is without effect on plasminogen activator activity in human granulosa lutein cell cultures.

The present findings suggest a pluripotent response of GLCs to IL-1, characterized by the induction of PAI-1 and the suppression of PA occurring concurrent with, but independent of, PG production.

Interleukin-1beta inhibits steroidogenic bioactivity in cultured rat ovarian granulosa cells by stimulation of progesterone degradation and inhibition of estrogen formation.

An IL-1beta-mediated inhibition of gonadotropin-stimulated steroidogenesis via modulation of specific enzymes is demonstrated, and a role for IL- 1beta is suggested in mediating the observed decline of these bioactive hormones during ovulation and luteolysis.

Evidence that granulosa cells inhibit interleukin-1α and interleukin-2 production from follicular lymphomonocytes

These results suggest that preovulation, a migration of lymphomonocytes from the peripheral compartment to the follicle occurs, and unfavorable effects of IL2 and IL1α, cytotoxic and antisteroidogenetic activities, are counteracted by the products of granulosa cells.



Interleukin-1 inhibits luteinization of porcine granulosa cells in culture.

It was shown for the first time that IL-1 can modulate steroidogenic functions of mammalian cells in culture and markedly inhibited the LH-stimulated progesterone production in a dose-dependent manner.

Human chorionic gonadotropin and prolactin modulation of early luteal function and luteinizing hormone receptor-binding activity in cultured human granulosa-luteal cells.

Results show that cultured human granulosa-luteal cells are responsive to hCG, with parallel increases in both progesterone production and [125I]hCG receptor binding.

Discordance in the effects of interleukin-1 on rat granulosa cell differentiation induced by follicle-stimulating hormone or activators of adenylate cyclase.

IL-1 appeared to inhibit forskolin-induced and cholera toxin-induced formation of LH receptors even when cAMP levels were elevated, while 8-bromo cAMP-stimulated progesterone secretion was significantly enhanced by IL-1, but LH receptor formation was inhibited.

Interleukin-1 stimulates human chorionic gonadotropin secretion by first trimester human trophoblast.

The results suggest that IL-1 may be an important local regulator of hCG secretion by first trimester trophoblasts as well as by the JAR cells.

Inhibitory actions of interleukin-1 beta on steroidogenesis in primary cultures of neonatal rat testicular cells.

The conversion of exogenously added androgen precursors to testosterone by human chorionic gonadotropin-stimulated cells was suppressed by interleukin-1 beta suggesting that the activity of the 17 alpha-hydroxylase enzyme may be decreased.

Inhibitory effects of interleukin-1 on luteinizing hormone-stimulated adenosine 3',5'-monophosphate accumulation by cultured porcine granulosa cells.

The results indicate that the inhibitory effect of IL-1 on LH- Stimulated progesterone secretion is due to its actions at at least two different sites along the LH-stimulated, cAMP-mediated, progester one biosynthetic pathway, the LH receptor level and adenylate cyclase systems.

Lymphokines from concanavalin-A-stimulated lymphocytes regulate rat granulosa cell steroidogenesis in vitro.

The present studies demonstrate that secretory products of lymphocytes (collectively termed lymphokines) can affect steroidogenesis in cultured rat granulosa cells, implying that immune cell factors may play a significant role in the differentiation and maturation of granULosa cells.

Cellular basis of luteal steroidogenesis in the human ovary.

Results show that luteal cell steroidogenesis becomes increasingly dependent upon gonadotrophic support with CL age, evidence that functional luteolysis in human ovaries is pre-programmed to occur at the cellular level and is initiated automatically at the time of ovulation.

The Biochemistry, Biology, and Role of Interleukin 2 in the Induction of Cytotoxic T Cell and Antibody‐Forming B Cell Responses

A broad spectrum of biological activities has been ascribed to IL 2, and the definitive biological assay for IL 2 measures the factor's ability to maintain the growth of factor-dependent cytotoxic T cell lines.