Lymphocytes of the toad Xenopus laevis have the gene set for promoting tadpole development

  title={Lymphocytes of the toad Xenopus laevis have the gene set for promoting tadpole development},
  author={Matthias R. Wabl and Rudolf B. Brun and Louis Du Pasquier},
  pages={1310 - 1312}
Nuclear transplantation experiments show that differentiated cells, such as lymphocytes, from the adult frog can express the genes necessary for tadpole development. The transplanted cells were proven to be lymphocytes by immunological methods. The origin of the tadpoles that developed after lymphocyte nuclei injections was ascertained by a karyotypic marker. 

Genomic multipotentiality of differentiated somatic cells.

  • M. Diberardino
  • Biology
    Cell differentiation and development : the official journal of the International Society of Developmental Biologists
  • 1988

Gene reactivation in erythrocytes: nuclear transplantation in oocytes and eggs of Rana.

These results demonstrate that nuclei of noncycling and terminally differentiated erythrocytes contain the genes to specify tadpole development, and conditioning these nuclei in the cytoplasm of oocytes leads to a widespread reactivation of dormant genes.

The current status of cloning and nuclear reprograming in amphibian eggs.

The process of cloning on our planet dates back to the initial reproduction of unicellular plant and animal forms and also to the propagation of multicellular forms by budding. Nuclear

Genomic potential of differentiated cells analyzed by nuclear transplantation

Results of nuclear transplantation experiments in amphibian oocytes and eggs suggest that the genetic totipotency of at least some differentiated somatic cell types still remains a tenable hypothesis.

Origin and progress of nuclear transfer in nonmammalian animals.

Classic studies in cytology, embryology, or genetics spanning more than five centuries that led to nuclear transfers in unicellular animals and to those in oocytes of insects, fish and amphibians are reviewed.

Nuclear transplantation in Bombina orientalis and utilization of the Pale mutation as a nuclear marker.

The first successful transplantation of embryonic nuclei into enucleated eggs of this organism is reported, demonstrating that nuclear transplantation in B. orientalis provides embryos whose nuclei may be reliably interpreted as being derived from the injected nucleus.

Transplantation of nuclei from lymphocytes of adult frogs into enucleated eggs: special focus on technical parameters.

The technique of transplantation of nuclei from adult lymphocytes into enucleated eggs from Xenopus laevis (South African clawed toad) shows that differentiated cells from adults, such as lymphocytes, can re-express the genes necessary for ontogeny.



The thymus during the ontogeny of the toad Xenopus laevis: Growth, membrane‐bound immunoglobulins and mixed lymphocyte reaction

The reactivity of thymic lymphocytes in the mixed lymphocyte reaction of the toad Xenopus laevis is already established in the 4th week after fertilization, and stability of the thymocyte reactivity contrasts with other aspects of theThymus development.

A description of the technique for nuclear transplantation in Xenopus laevis.

Differences between the eggs of Rana and Xenopus have made it necessary to modify the technique before it can be satisfactorily applied to the Eggs of Xenopus, and the extent to which these modifications might affect a direct comparison of the results of transplantation experiments is discussed.

Transplantation of Living Nuclei From Blastula Cells into Enucleated Frogs' Eggs.

  • R. BriggsT. J. King
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1952
The role of the nucleus in embryonic differentiation has been the subject of investigations dating back to the beginnings of experimental embryology, and the known cytogenetical mechanisms that could account for nuclear differentiation have been indicated.

Methods of transplanting nuclei from single cultured cells to unfertilized frogs' eggs.

SUMMARY Two methods of transplanting single nuclei from monolayers of cultured cells to unfertilized eggs of Xenopus laevis are described, illustrated, and tested. The detached-cell method is simpler

The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs.

It is concluded that cell specialization does not involve any loss, irreversible inactivation or permanent change in chromosomal genes required for development.

Direct evidence for immunoglobulins on the surface of thymus lymphocytes of amphibian larvae

There is a sharp increase in the level of lymphocytes carrying immunoglobulins in the thymus at very early stages of their development, and this level remains constant during the entire larval and early adult life.

Antigen-binding cells. I. Their idenification and role in the immune response.

  • E. Unanue
  • Biology, Medicine
    Journal of immunology
  • 1971
Incubation of spleen cells from immune or non-immune mice with highly radioactive KLH in vitro suppressed the capacity of the cells to adaptively transfer an immune response in x-irradiated mice.

Binding and release of lymphocytes by hapten‐derivatized nylon fibers

Experiments suggest that capping of the lymphocytes is involved in their release from the fibers, and that cells bound at 4 °C for more than 12 h can be released quantitatively from the fiber at temperatures above 22 °C within 2 h in a fully viable state.

Cell fractionation and arrangement on fibers, beads, and surfaces.

A new method, fiber fractionation, has been used to isolate and separate cells, and beads and surfaces have been specifically derivatized and used to achieve different geometrical arrangements of the cells.