Lymphocyte stimulation: a rapid multiparameter analysis.

  title={Lymphocyte stimulation: a rapid multiparameter analysis.},
  author={Zbigniew Darzynkiewicz and Frank Traganos and Thomas K. Sharpless and Myron R. Melamed},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={73 8},
Several parameters of stimulation of individual lymphocytes are measured simultaneously by flow-cytofluorometry after differential staining of cellular DNA and RNA with the metachromatic fluorescent dye acridine orange. The method provides a means of analyzing the progression of stimulated cells through the cell cycle (G1, S and G2 + M), in addition to measuring the accumulation of RNA per cell; the RNA parameter is useful in distinguishing G1 from G0 cells. The multiparameter analysis may be… CONTINUE READING

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