Lymphocyte blastogenesis studied by volume spectroscopy.

Abstract

The blastogenesis of human blood lymphocytes has been studied by measuring the distribution of cellular and nuclear volumes in concanavalin A (Con A)-stimulated leucocyte cultures by means of a modified Coulter counter. Agglutinated and adherent cells were dispersed by 3 h of incubation with alpha-methyl-mannoside and EDTA. Fresh suspensions contained 5--35… (More)

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