Lymphocyte-Dependent Inhibition of Growth of Virulent Mycobacterium tuberculosis H 37 Rv Within Human Monocytes : Requirement for CD 4 1 T Cells in Purified Protein Derivative-Positive , But Not in Purified Protein Derivative-Negative Subjects 1

Protective human immunity to Mycobacterium tuberculosis (M. tb) has proven difficult to characterize, in part because of technical obstacles to in vitro infection of human cells with virulent M. tb. We established a reproducible method of infecting human monocytes (MN) with the virulent M. tb strain H37Rv that did not reduce MN viability. TNF-a had no… (More)