Lyme borreliosis — Problems of serological diagnosis

@article{Hofmann2005LymeB,
  title={Lyme borreliosis — Problems of serological diagnosis},
  author={Heidelore Hofmann},
  journal={Infection},
  year={2005},
  volume={24},
  pages={470-472}
}
  • H. Hofmann
  • Published 1 November 1996
  • Medicine
  • Infection
SummaryAs long as test procedures are not standardized, the serological results of IgM-and IgG-antibodies in Lyme borreliosis must be interpreted with caution and always in the context of clinical signs and symptoms. False negative results occur primarily during the first weeks of infection. In erythema migrans of less than 4 weeks' duration, 50% of patients are seronegative even with newly designed ELISAs. At this early stage of the infection the therapeutic decision has to be established on… 

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References

SHOWING 1-8 OF 8 REFERENCES

Microbiological diagnosis in Lyme borreliosis.

  • B. Wilske
  • Biology, Medicine
    International journal of medical microbiology : IJMM
  • 2002
TLDR
For the microbiological diagnosis of Lyme borreliosis, antibody detection methods are mainly used and progress has been made in the sensitivity of the recombinant blot using additional antigens as p58 and homologues of Osp17.

Western blotting in the serodiagnosis of Lyme disease.

TLDR
Western blotting can be used to increase the specificity of serologic testing in Lyme disease.

Immunoblot interpretation criteria for serodiagnosis of early Lyme disease.

TLDR
The antibody responses of 55 treated patients with early Lyme disease and physician-documented erythema migrans were monitored and a relational database management system was used to analyze the results and provide criteria for early disease immunoblot interpretation.

Anxiety and persistence of Lyme disease.

  • L. Sigal
  • Medicine, Psychology
    The American journal of medicine
  • 1995

Recommendations for test performance and interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease.

  • Medicine
    MMWR. Morbidity and mortality weekly report
  • 1995
TLDR
This report presents recommendations for serologic test performance and interpretation, which included substantial changes in the recommended tests and their interpretation for the serodiagnosis of LD.

Serodiagnosis of European skin borreliosis and localized scleroderma with different ELISAs and Immunoblots

  • VII International Congress on Lyme Borreliosis. San Francisco
  • 1996

Serological follow-up with two ELISA systems after treatment of skin borreliosis

  • VII International Congress on Lyme Borreliosis,
  • 1996

Microbiological Diagnosis of Lyme Borreliosis Test performance and interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease

  • MMWR Morb . Mortal . Wkly . Rep .
  • 1995