Lycopene-family carotenoids confer thermostability on photocomplexes from a new thermophilic purple bacterium.

  title={Lycopene-family carotenoids confer thermostability on photocomplexes from a new thermophilic purple bacterium.},
  author={Ryuta Seto and Shinichi Takaichi and T Kurihara and Rikako Kishi and Mai Honda and Shinji Takenaka and Yusuke Tsukatani and Michael T. Madigan and Zheng-Yu Wang-Otomo and Yukihiro Kimura},
Blastochloris (Blc.) tepida is a newly described thermophilic purple bacterium containing bacteriochlorophyll (BChl) b. Using purified light-harvesting 1 reaction center (LH1-RC) core complexes from Blc. tepida, we compared the biochemical, spectroscopic, and thermal denaturation properties of these complexes with those of its mesophilic counterpart, Blc. viridis. Besides their growth temperature optima, a striking difference between the two species was seen in the carotenoid composition of… 

An LH1–RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins

Comparison of the N-terminal regions of the Cyt with PufX polypeptides widely distributed in Rhodobacter species reveals significant structural similarities, supporting a longstanding hypothesis that Puf X is phylogenetically related to the N theterminus of the RC-bound Cyt subunit.

Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes

Results of circular dichroism and resonance Raman measurements on the purified LH1 complexes in a reaction center-associated form from a mesophilic and a thermophilic Blastochloris species provide crucial insights into the spectral and structural origins for the ultra-redshift of the long-wavelength absorption maximum of BChl b-containing phototrophs.

Advances in the Spectroscopic and Structural Characterization of Core Light-Harvesting Complexes from Purple Phototrophic Bacteria.

Purple phototrophic bacteria are ancient anoxygenic phototrophs and attractive research tools because they capture light energy in the near-infrared (NIR) region of the spectrum and transform it into

An Overview on Industrial and Medical Applications of Bio-Pigments Synthesized by Marine Bacteria

Light is shed on marine bacterial sources of pigmented compounds along with their industrial applicability and therapeutic insights based on the data available in the literature, and the need for introducing bacterial bio-pigments in global pigment industry is encompassed.

A Ca 2+ -binding motif underlies the unusual properties of certain photosynthetic bacterial core light-harvesting complexes

The mildly thermophilic purple phototrophic bacterium Allochromatium tepidum provides a unique model for inves-tigating and can be used as a model for future generations of antibiotics.



Effects of Calcium Ions on the Thermostability and Spectroscopic Properties of the LH1-RC Complex from a New Thermophilic Purple Bacterium Allochromatium tepidum.

The light harvesting-reaction center (LH1-RC) complex from a new thermophilic purple sulfur bacterium Allochromatium (Alc.) tepidum was isolated and characterized by spectroscopic and thermodynamic

Preferential Incorporation of Coloured-carotenoids Occurs in the LH2 Complexes From Non-sulphur Purple Bacteria Under Carotenoid-limiting Conditions

The results show that although the presence of Car molecules is important for the stability of the LH2 complexes that the overall native structure can be maintained by the absence of many different Cars.

Carotenoid-induced cooperative formation of bacterial photosynthetic LH1 complex.

The extension of the conjugation size of the antenna carotenoids provides a mechanism for the spectral tuning of light harvesting in the visible part of the spectrum with characteristically red-shifted absorption and emission maxima.

Structure analysis and comparative characterization of the cytochrome c' and flavocytochrome c from thermophilic purple photosynthetic bacterium Thermochromatium tepidum.

The thermodynamic and spectroscopic properties of two soluble electron transport proteins, cytochrome (Cyt) c' and flavocytochrome c, isolated from thermophilic purple sulfur bacterium

A Dual Role for Ca2+ in Expanding the Spectral Diversity and Stability of Light-Harvesting 1 Reaction Center Photocomplexes of Purple Phototrophic Bacteria.

The light-harvesting 1 reaction center (LH1-RC) complex in the purple sulfur bacterium Thiorhodovibrio ( Trv.) strain 970 cells exhibits its LH1 Q y transition at 973 nm, the lowest-energy Q y

Electronic properties and thermal stability of soluble redox proteins from a thermophilic purple sulfur photosynthetic bacterium, Thermochromatium tepidum

The HiPIP was confirmed to be the possible electron-transfer protein to the reaction center under physiological conditions and is thermally stable up to 60 °C, which is much higher than that of the mesophilic photosynthetic bacterium, Allochroimitiiim (A.) vinosum.

Spectroscopic and Thermodynamic Characterization of the Metal-Binding Sites in the LH1-RC Complex from Thermophilic Photosynthetic Bacterium Thermochromatium tepidum.

The present results are largely compatible with the recent structural evidence that another binding site for Sr2+ (or Ba2+) exists in the vicinity of the Ca2+-binding site, a part of which is shared in both metal-binding sites.