Lung inflammation after bleomycin treatment in mice: Selection of an accurate normalization strategy for gene expression analysis in an ex-vivo and in-vitro model.

@article{Latta2017LungIA,
  title={Lung inflammation after bleomycin treatment in mice: Selection of an accurate normalization strategy for gene expression analysis in an ex-vivo and in-vitro model.},
  author={Veronica Della Latta and M. Cabiati and Silvia Burchielli and Giada Frenzilli and Margherita Bernardeschi and Antonella Cecchettini and F. Viglione and M A Morales and Silvia del Ry},
  journal={The international journal of biochemistry & cell biology},
  year={2017},
  volume={88},
  pages={145-154}
}
Pulmonary fibrosis (PF) is the most common and aggressive interstitial lung disease, characterized by a patchy development of fibrosis leading to progressive destruction of the normal lung architecture which is preceded by an inflammatory process. Gene expression studies are important to understand the development of PF but the accuracy and reproducibility of Real-Time PCR depend on appropriate normalization strategies. This study aimed to analyze the expression variability of eight commonly… CONTINUE READING