Lung epithelial cell lines in coculture with human pulmonary microvascular endothelial cells: development of an alveolo-capillary barrier in vitro

  title={Lung epithelial cell lines in coculture with human pulmonary microvascular endothelial cells: development of an alveolo-capillary barrier in vitro},
  author={Maria Iris Hermanns and Ronald E. Unger and Kai Kehe and Kirsten Peters and Charles James Kirkpatrick},
  journal={Laboratory Investigation},
We have established a coculture system of human distal lung epithelial cells and human microvascular endothelial cells in order to study the cellular interactions of epithelium and endothelium at the alveolocapillary barrier in both pathogenesis and recovery from acute lung injury. The aim was to determine conditions for the development of functional cellular junctions and the formation of a tight epithelial barrier similar to that observed in vivo. The in vitro coculture system consisted of… 
A coculture model of the lung–blood barrier: the role of activated phagocytic cells.
Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability
Altered tight junction permeability in cocultures indicates that these cells can work together as an active part of the mucosal barrier.
Primary human coculture model of alveolo-capillary unit to study mechanisms of injury to peripheral lung
The established coculture provides a suitable in vitro model to examine barrier function at the distal lung, including the interaction of microvascular endothelial cells with ATII-like and ATI-like epithelial cells.
Human alveolar epithelial cells expressing tight junctions to model the air-blood barrier.
The results suggest that hAELVi cells reflect the essential features of the air-blood barrier, as needed for an alternative to animal testing to study absorption and toxicity of inhaled drugs, chemicals and nanomaterials.
Barrier functions and paracellular integrity in human cell culture models of the proximal respiratory unit.
  • C. Pohl, M. Hermanns, C. Kirkpatrick
  • Biology
    European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
  • 2009
An in vitro triple cell co-culture model with primary cells mimicking the human alveolar epithelial barrier.
Human Co- and Triple-Culture Model of the Alveolar-Capillary Barrier on a Basement Membrane Mimic.
This in vitro model consisting of epithelial, endothelial, and immune cells on a basement membrane mimic was able to maintain adequately the barrier properties of the bipolar coculture, thus making it a promising basis for further studies in tissue engineering.
The cell line NCl-H441 is a useful in vitro model for transport studies of human distal lung epithelial barrier.
NCl-H441 cells are the first cell line of human distal lung epithelial origin with the ability to form monolayers with appreciable barrier properties and drug transporter expression and activity in NCl- H441 cells was consistent with what has been reported for human alveolar epithelial cells in primary culture.


Monolayers of Human Alveolar Epithelial Cells in Primary Culture for Pulmonary Absorption and Transport Studies
A primary cell culture model of human alveolar epithelial cells has been established, which appears to be a valuable in vitro model for pulmonary drug delivery and transport studies.
Tightening of endothelial cell contacts: a physiologic response to cocultures with smooth-muscle-like 10T1/2 cells.
Mural cells such as smooth muscle cells and pericytes may be important for stabilizing endothelial cell-to-cell contacts and may influence vessel-type specific differences of the endothelial phenotype.
In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R.
HPMEC-ST1.6R will be a valuable in vitro model system in which to study pathomechanisms and angiogenesis of the mature microvascular endothelium in vitro.
Comparative study of adhesion molecule expression in cultured human macro- and microvascular endothelial cells.
The study suggests that E-selectin- and VCAM-coated beads could potentially serve in the isolation process of arteriolar and venular ECs, which are only partially transferable to in vivo conditions.
Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism.
The results indicated that the A549 cell line may be useful for the studying the metabolic and macromolecule processing contributions of alveolar Type II cells to mechanisms of drug delivery at the pulmonary epithelium.
Monocyte Migration Through the Alveolar Epithelial Barrier: Adhesion Molecule Mechanisms and Impact of Chemokines1
Unlike transendothelial monocyte traffic, for which β1 and β2 integrins are alternative mechanisms, monocyte migration across the alveolar epithelium largely depends on CD11b/CD18 and CD47 but required the additional engagement of the β1 Integrins for optimal migration.
Type II pneumocytes in mixed cell culture of human lung: a light and electron microscopic study.
This study proposes this as a useful model for the study of human Type II epithelial cells in vitro and proposes that free collagen fibers, present in the original preparation, spindle cells, and/or Type II cells could be responsible for presence of ECM.
Structural and functional aspects of intercellular junctions in vascular endothelium
The organization and functional aspects of the occludin/ZO-1 complex typically found in tight junctions, the cadherin/catenin complex of the adherens junctions and the connection of these protein complexes to the dense peripheral band via actin filaments are described.
H441 pulmonary epithelial cell mitogenic effects and signaling pathways in response to HGF and TGF-alpha.
H441 cells exhibited responses to mitogenic growth factors similar to type II cells and may be useful as a model for type II cell growth factor responses and signal transduction.
Lung fibroblasts improve differentiation of rat type II cells in primary culture.
The observations suggest that epithelial--mesenchymal interactions affect type II cell differentiation in the adult lung, and that these effects are partially mediated by KGF.