Luminescence immunoassay of pollen allergens on air sampling polytetrafluoroethylene filters.

@article{Holmquist1999LuminescenceIO,
  title={Luminescence immunoassay of pollen allergens on air sampling polytetrafluoroethylene filters.},
  author={Leif Holmquist and Olof Vesterberg},
  journal={Journal of biochemical and biophysical methods},
  year={1999},
  volume={41 1},
  pages={49-60}
}
A new method for quantification of airborne birch and grass pollen allergens collected on porous polytetrafluoroethylene filters has been developed. In this method, the allergens firmly adsorbed to the sampling filter of 25 mm in diameter are reacted with specific antibodies conjugated to alkaline phosphatase, generating a matrix-bound allergen-antibody-phosphatase complex. The filter is then floated on a chemiluminescent enzyme substrate solution. The light intensity of the product is linearly… CONTINUE READING