Luciferase from the Italian firefly Luciola italica: molecular cloning and expression.

@article{Branchini2006LuciferaseFT,
  title={Luciferase from the Italian firefly Luciola italica: molecular cloning and expression.},
  author={B. Branchini and Tara L. Southworth and Jennifer P. DeAngelis and A. Roda and E. Michelini},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={2006},
  volume={145 2},
  pages={
          159-67
        }
}
The cDNA encoding the luciferase from the Italian firefly Luciola italica was cloned using reverse transcriptase-PCR and a gene-specific primer set based on the DNA sequence of Luciola mingrelica. The cDNA sequence of L. italica luciferase was determined to be 1647 base pairs in length with an open reading frame of 548 amino acids. Phylogenetic analysis of the protein sequence demonstrated that this luciferase is closely related to that of other fireflies of the Lampyridae family, particularly… Expand
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